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Your Position: Home > All Other Proteins > Siglec-9 > SI9-H52H4

Human Siglec-9 Protein, His Tag

  • Synonym
    Siglec-9,SIGLEC9,CDw329,CD329
  • Source
    Human Siglec-9, His Tag (SI9-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 348 (Accession # Q9Y336-1).
    Predicted N-terminus: Gln 18
  • Molecular Characterization
    Online(Gln 18 - Gly 348) Q9Y336-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 37.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-9, His Tag (Cat. No. SI9-H52H4) SDS-PAGE gel

Human Siglec-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human Siglec-9, His TagHuman Siglec-9, His Tag (Cat. No. SI9-H52H4) ELISA bioactivity

Immobilized Human Siglec-9, His Tag (Cat. No. SI9-H52H4) at 10 μg/mL (100 μL/well) on Nickel Coated plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-2.5 μg/mL (QC tested).

  • Background
    siglec-9 (HGMW-approved symbol SIGLEC9) a member of the sialic acid-binding Ig-like lectin (Siglec) family, which belongs to the immunoglobulin superfamily (IgSF). SIGLEC9 shows a high degree of homology to many members of the siglec family, including siglec-7 (80%), siglec-8 (72%), siglec-5 (65%), and CD33 (64%). This high degree of homology is also conserved in the extracellular Ig-like domains. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains。Siglec-9 with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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