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Your Position: Home > All Other Proteins > Siglec-9 > SI9-H5256

Human Siglec-9 Protein, Fc Tag

  • Synonym
    Siglec-9,SIGLEC9,CDw329,CD329
  • Source
    Human Siglec-9, Fc Tag (SI9-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 348 (Accession # Q9Y336-1).
    Predicted N-terminus: Gln 18
  • Molecular Characterization
    Online(Gln 18 - Gly 348) Q9Y336-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 62.5 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-9, Fc Tag (Cat. No. SI9-H5256) SDS-PAGE gel

Human Siglec-9, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Siglec-9, Fc TagHuman Siglec-9, Fc Tag (Cat. No. SI9-H5256) ELISA bioactivity

Immobilized Human Siglec-9, Fc Tag (Cat. No. SI9-H5256) at 10 μg/mL (100 μL/well) on Diamond Protein A Protein, His Tag precoated (0.5 μg/well) plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.156-2.5 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human Siglec-9, Fc Tag (Cat. No. SI9-H5256) on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0.85 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    siglec-9 (HGMW-approved symbol SIGLEC9) a member of the sialic acid-binding Ig-like lectin (Siglec) family, which belongs to the immunoglobulin superfamily (IgSF). SIGLEC9 shows a high degree of homology to many members of the siglec family, including siglec-7 (80%), siglec-8 (72%), siglec-5 (65%), and CD33 (64%). This high degree of homology is also conserved in the extracellular Ig-like domains. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains。Siglec-9 with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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