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Your Position: Home > All Other Proteins > PSMA > PSA-HF244

FITC-Labeled Human PSMA / FOLH1 Protein, His Tag

  • Synonym
    FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP
  • Source
    FITC-Labeled Human PSMA, His Tag (PSA-HF244) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # Q04609-1). It is the FITC labeled form of Human PSMA, His Tag (PSA-H52H3).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Lys 44 - Ala 750) Q04609-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 81.4 KDa. The protein migrates as 85-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1-3.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 25 mM MES, 500 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human PSMA, His Tag (Cat. No. PSA-HF244) SDS-PAGE gel

FITC-Labeled Human PSMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
FITC-Labeled Human PSMA, His TagFITC-Labeled Human PSMA, His Tag (Cat. No. PSA-HF244) ELISA bioactivity

Immobilized Monoclonal Anti-Human PSMA Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human PSMA, His Tag (Cat. No. PSA-HF244) with a linear range of 0.156-1.25 μg/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-PSMA CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human PSMA Protein, His Tag (Cat. No. PSA-HF244) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is stable at pH greater than 6.5. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. FOLH1 / GCP-2 is homodimer and binds 2 zinc ions per subunit, and required for NAALADase activity. The catalytic activity of PSMA involved in releasing of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly – gamma - glutamates. FOLH1 / GCP-2 / PSMA has both folate hydrolase and N – acetylated – alpha – linked - acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. GCP-2 / PSMA involved in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $319.00

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