Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.9 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 45-55 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin with an affinity constant of 3.42 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) captured on Biotin CAP-Series S Sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 2.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) on SA Biosensor, can bind Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) with an affinity constant of 0.495 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Kielau Kisalu, Neville et al.
Journal: JCI Insight 2020
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