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Your Position: Home > Cytokines > IL-13 > IL3-H5256

Human IL-13 Protein, Fc Tag

  • Synonym
    IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13
  • Source
    Human IL-13, Fc Tag (IL3-H5256) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).
    Predicted N-terminus: Gly 21
  • Molecular Characterization
    Online(Gly 21 - Asn 132) AAK53823.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 38.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-13, Fc Tag (Cat. No. IL3-H5256) SDS-PAGE gel

Human IL-13, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human IL-13, Fc TagHuman IL-13, Fc Tag (Cat. No. IL3-H5256) ELISA bioactivity

Immobilized Human IL-13, Fc Tag (Cat. No. IL3-H5256) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-13 Antibody, Human IgG4 with a linear range of 0.4-6 ng/mL (QC tested).

Human IL-13, Fc TagHuman IL-13, Fc Tag (Cat. No. IL3-H5256) ELISA bioactivity

Immobilized Human IL-13, Fc Tag (Cat. No. IL3-H5256) at 5 μg/mL (100 μL/well) can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human IL-13, Fc Tag (Cat. No. IL3-H5256) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1980.00

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