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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 37.9 kDa. The protein migrates as 50 kDa and 55-85 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CADM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Cynomolgus CRTAM, Fc Tag (Cat. No. CRM-C5255) at 5 μg/mL (100 μL/well) can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with a linear range of 0.6-5 ng/mL (QC tested).
Loaded Human CRTAM, His Tag (Cat. No. CRM-H52H3) on AR2G Biosensor, can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with an affinity constant of 51.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Cynomolgus CRTAM, Fc Tag (Cat. No. CRM-C5255) on Protein A Biosensor, can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with an affinity constant of 37.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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