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Your Position: Home > Receptor Tyrosine Kinases > ErbB3 > ER3-H5257

Human ErbB3 / Her3 Protein, Mouse IgG2a Fc Tag (MALS verified)

  • Synonym
    ERBB3,HER3,LCCS2,MDA-BF-1,MGC88033,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3
  • Source
    Human ErbB3, Mouse IgG2a Fc Tag (ER3-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # P21860-1).
    Predicted N-terminus: Ser 20
  • Molecular Characterization
    Online(Ser 20 - Thr 643) P21860-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 95.5 kDa. The protein migrates as 115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ErbB3, Mouse IgG2a Fc Tag (Cat. No. ER3-H5257) SDS-PAGE gel

Human ErbB3, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human ErbB3, Mouse IgG2a Fc Tag (Cat. No. ) MALS images

The purity of Human ErbB3, Mouse IgG2a Fc Tag(Cat. No. ER3-H5257) was more than 90% and the molecular weight of this protein is around 220-260 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human ErbB3, Mouse IgG2a Fc TagHuman ErbB3, Mouse IgG2a Fc Tag (Cat. No. ER3-H5257) ELISA bioactivity

Immobilized Human NRG1 Beta 1, Fc Tag (Cat. No. NR1-H5268) at 2 μg/mL (100 μL/well) can bind Human ErbB3, Mouse IgG2a Fc Tag (Cat. No. ER3-H5257) with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:18 Details
  • Latest Research Phase:Phase 3 Clinical

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