This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 37.9 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human FGL1, His,Avitag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Loaded Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) on SA Biosensor, can bind Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) with an affinity constant of 8.12 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS assay shows that Biotinylated Human FGL1, His,Avitag (Cat. No. FG1-H82Ey) can bind to 293T cells overexpressing Human LAG3. The concentration of Human FGL1 is 10 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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