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Product Details
Synonyms
FGL1, Hepassocin, HP-041, HFREP-1, LFIRE-1, HFREP1
Source
Biotinylated Human FGL1 Protein, His,Avitag (FG1-H82Ey) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Ile 312 (Accession # Q08830-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 37.9 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human FGL1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate can bind Anti-FGL1 antibody with a linear range of 1-40 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human FGL1 Protein, His,Avitag (Cat. No. FG1-H82Ey) on SA Biosensor, can bind Human LAG-3 Protein, Fc Tag (Cat. No. LA3-H5255) with an affinity constant of 8.12 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
FACS assay shows that Biotinylated Human FGL1 Protein, His,Avitag (Cat. No. FG1-H82Ey) can bind to 293T cells overexpressing Human LAG3. The concentration of Human FGL1 is 10 μg/mL (Routinely tested).
Protocol
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