This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 41.5 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
FITC-Labeled Human CD4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, His Tag (Cat. No. CD4-HF2H7) with a linear range of 0.01-0.156 μg/mL (QC tested).
Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, His Tag (Cat. No. CD4-HF2H7) with a linear range of 0.039-0.625 μg/mL (Routinely tested).
2e5 of CD4-CAR-293 cells were stained with 100 μL of 0.3 μg/mL of FITC-Labeled Human CD4, His Tag (Cat. No. CD4-HF2H7) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $319.00
Price(USD) : $2180.00
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