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Your Position: Home > All Other Proteins > Mucin-1 > MU1-H82E5

Biotinylated Human Mucin-1 (890-1158) Protein, His,Avitag™

  • Synonym
    Mucin 1,MUC1,CD227,EMA,H23AG,KL-6,MAM6,MUC-1,SEC,MUC-1,X,MUC1,ZD,PEM,PEMT,PUM,CA15-3,Episialin
  • Source
    Biotinylated Human Mucin-1 (890-1158), His,Avitag (MU1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ser 890 - Gly 1158 (Accession # P15941-1).
    Predicted N-terminus: Ser 890 (partial α chain) & Ser 1098 (partial β chain)
  • Molecular Characterization
    Online(Ser 890 - Gly 1158) P15941-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 21.3 kDa (partial α chain) and 10.2 kDa (partial β chain). The protein migrates as 45-70 kDa (partial α chain) and 7 kDa and 14-15 kDa (partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Mucin-1 (890-1158), His,Avitag (Cat. No. MU1-H82E5) SDS-PAGE gel

Biotinylated Human Mucin-1 (890-1158), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-Mucin-1 CAR-293 cells were stained with 100 μL of 0.1 μg/mL of Biotinylated Human Mucin-1, His,Avitag (Cat. No. MU1-H82E5) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    Mucin-1 (MUC1) is also known as Tumor-associated epithelial membrane antigen (EMA), Polymorphic epithelial mucin (PEM), Peanut-reactive urinary mucin (PUM), PEMT, Krebs von den Lungen-6 (KL-6), CD antigen CD227, Episialin, H23AG. MUC1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. Mucins protect the body from infection by pathogen binding to oligosaccharides in the extracellular domain, preventing the pathogen from reaching the cell surface. Except protective function by binding to pathogens, MUC1 also functions in a cell signaling capacity. Overexpression of MUC1 is often associated with colon, breast, ovarian, lung and pancreatic cancers.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:16 Details
  • Latest Research Phase:Phase 3 Clinical

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DMF (Drug Master File)

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