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Your Position: Home > All Other Proteins > Mucin-1 > MU1-H82E5

Biotinylated Human Mucin-1 (890-1158) Protein, His,Avitag™

  • Synonym
    Mucin 1,MUC1,CD227,EMA,H23AG,KL-6,MAM6,MUC-1,SEC,MUC-1,X,MUC1,ZD,PEM,PEMT,PUM,CA15-3,Episialin
  • Source
    Biotinylated Human Mucin-1 (890-1158), His,Avitag (MU1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ser 890 - Gly 1158 (Accession # P15941-1).
    Predicted N-terminus: Ser 890 (partial α chain) & Ser 1098 (partial β chain)
  • Molecular Characterization
    Online(Ser 890 - Gly 1158) P15941-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 21.3 kDa (partial α chain) and 10.2 kDa (partial β chain). The protein migrates as 45-70 kDa (partial α chain) and 7 kDa and 14-15 kDa (partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Mucin-1 (890-1158), His,Avitag (Cat. No. MU1-H82E5) SDS-PAGE gel

Biotinylated Human Mucin-1 (890-1158), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-Mucin-1 CAR-293 cells were stained with 100 μL of 0.1 μg/mL of Biotinylated Human Mucin-1, His,Avitag (Cat. No. MU1-H82E5) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. Interacts via its C-terminus with PDZK1 and this interaction appears important for proper localization. Probably plays a role in maintaining homeostasis on mucosal surfaces.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:16 Details
  • Latest Research Phase:Phase 3 Clinical

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