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Your Position: Home > All Other Proteins > Integrin alpha 1 beta 1 > IT1-H52Wb

Human Integrin alpha 1 beta 1 (ITGA1&ITGB1) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    Integrin alpha 1 beta 1,ITGA1&ITGB1
  • Source
    Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52Wb) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Pro 1141 (ITGA1) & Gln 21 - Asp 728 (ITGB1) (Accession # P56199-1 (ITGA1) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 29 (ITGA1) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Phe 29 - Pro 1141 (ITGA1) & Gln 21 - Asp 728 (ITGB1)) P56199-1 (ITGA1) & P05556-1 (ITGB1)

    Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 170-200 kDa (ITGA1) and 100-130 kDa (ITGB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wb) SDS-PAGE gel

Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag FreeHuman ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wb) ELISA bioactivity

Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wb) with a linear range of 1-20 ng/mL (QC tested).

  • Background
    Integrin alpha-1/beta-1 is a receptor for laminin and collagen. The Integrin alpha-1/beta-1 and Integrin alpha-2/beta-1 integrins provide critical support for vascular endothelial growth factor signaling, endothelial cell migration, and tumor angiogenesis. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $410.00

Price(USD) : $2880.00

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