Human ALK-7 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
AL7-H52H5-100ug
$370.00
AL7-H52H5-1mg (500ug X 2)
$2580.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    ALK-7, ACTR-IC, ACVR1C

  • Source

    Human ALK-7, His Tag (AL7-H52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Glu 113 (Accession # Q8NER5-1).

    Predicted N-terminus: Glu 21

    Request for sequence
  • Molecular Characterization

    ALK-7 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 11.9 kDa. The protein migrates as 15-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ALK-7 SDS-PAGE

    Human ALK-7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    ALK-7 SEC-MALS

    The purity of Human ALK-7, His Tag (Cat. No. AL7-H52H5) is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ALK-7 ELISA

    Immobilized Human ALK-7, His Tag (Cat. No. AL7-H52H5) at 1 μg/mL (100 μL/well) can bind Rabbit anti-Homo sapiens (Human) ACVR1C Polyclonal antibody, Rabbit IgG with a linear range of 0.0005-0.125 μg/mL (QC tested).

    Protocol

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Background

ALK-7 (Activin receptor-like kinase 7) is also known as Activin receptor type-1C, ACTR-IC, ACVR1C. Serine/threonine protein kinase which forms a receptor complex on ligand binding. The receptor complex consisting of 2 type II and 2 type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators, SMAD2 and SMAD3. Receptor for activin AB, activin B and NODAL. Plays a role in cell differentiation, growth arrest and apoptosis.

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