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Your Position: Home > All Other Proteins > EphA4 > Human EphA4 Protein, His Tag

Human EphA4 Protein, His Tag

  • Synonym
    EphA4,HEK8,SEK,TYRO1,EK8
  • Source
    Human EphA4, His Tag (EP4-H52H8) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Thr 547 (Accession # P54764-1).
    Predicted N-terminus: Val 20
  • Molecular Characterization
    Online(Val 20 - Thr 547) P54764-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human EphA4, His Tag (Cat. No. EP4-H52H8) SDS-PAGE gel

Human EphA4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human EphA4, His TagHuman EphA4, His Tag (Cat. No. EP4-H52H8) ELISA bioactivity

Immobilized Human EphA4, His Tag (Cat. No. EP4-H52H8) at 5 μg/mL (100 μL/well) can bind Human Ephrin-A4, Fc Tag (Cat. No. EA4-H5250) with a linear range of 0.6-5 ng/mL (QC tested).

  • Background
    Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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