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Your Position: Home > All Other Proteins > IL-3 R alpha > ILA-M52H4

Mouse IL-3 R alpha / CD123 Protein, His Tag (SPR verified)

  • Synonym
    IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha
  • Source
    Mouse IL-3 R alpha, His Tag (ILA-M52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 17- Lys 331 (Accession # P26952-1).
    Predicted N-terminus: Ser 17
  • Molecular Characterization
    Online(Ser 17- Lys 331) P26952-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.4 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) SDS-PAGE gel

Mouse IL-3 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) captured on CM5 Chip via anti-His antibody can bind Mouse IL-3, Tag Free with an affinity constant of 0.704 μM as determined in SPR assay (Biacore T200) (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) on NTA Biosensor, can bind Mouse IL-3, Tag Free with an affinity constant of 0.38 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $300.00

Price(USD) : $1980.00

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