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Your Position: Home > Protein > Integrin alpha V beta 1 > IT1-H5213

Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, Tag Free&Tag Free

  • Synonym
    Integrin alpha V beta 1,ITGAV&ITGB1
  • Source
    Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free (IT1-H5213) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1)) NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)

    Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.3 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV contains no tag but an acidic tail at the C-terminus and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGAV) and 110-120 kDa (ITGB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free (Cat. No. IT1-H5213) SDS-PAGE gel

Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag FreeHuman ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free (Cat. No. IT1-H5213) ELISA bioactivity

Immobilized Recombinant Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free (Cat. No. IT1-H5213) with a linear range of 0.002-0.313 μg/mL (QC tested).

  • Background
    Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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