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Product Details
Synonyms
FOLR-1, FBP, FOLR, FRα
Source
Human FOLR1, His Tag (FO1-H52H1) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).
Predicted N-terminus: Arg 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human FOLR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human FOLR1, His Tag (Cat. No. FO1-H52H1) is more than 95% and the molecular weight of this protein is around 34-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Folic Acid-BSA Conjugate at 5 μg/mL (100 μL/well) can bind Human FOLR1, His Tag (Cat. No. FO1-H52H1) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Immobilized Human FOLR1, His Tag (Cat. No. FO1-H52H1) at 1 μg/mL (100 μL/well) can bind Anti-FOLR1 Antibody,Mouse IgG1 with a linear range of 0.2-1 ng/mL (QC tested).
Protocol
Immobilized Anti-FOLR1 Antibody,Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human FOLR1, His Tag (Cat. No. FO1-H52H1) with a linear range of 1-5 ng/mL (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of Anti-FOLR1 CAR-293 cells were stained with 100 μL of 0.3 μg/mL of Human FOLR1, His Tag (Cat. No. FO1-H52H1) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).
Protocol
Customer Reviews Writing Reviews
p***g2025-01-23
LikesAs expected, this was a high quality product. I have purchased multiple products from Acro to develop assays in the past. As usual, purity and quantity was confirmed by our in-house quality control group. We are using this in conjunction with #FO1-H82E2 Biotinylated Human FOLR1 Protein, His Avitag. The product was used successfully in an immunization campaign and will also be used for other in-house assays in the future.- D***m2022-10-26 LikesWe had to order human folate receptor protein for 100 micrograms unit type for one of our sensitive studies. It was crucial that the product work as intended. We received the product in a timely manner, and the product work as intended for one of our studies. As an employee working at a lab, I thank you for all the scientists working hard to make great products!
D***n2022-10-26 LikesWe had previously ordered this product and we made the mistake of ordering the smaller quantity needed. We contacted customer support letting them know that we needed to switch the order to the larger quantity. They were friendly and helpful when resolving this issue. After the switch, the item in question was sent out very quickly and we were able to continue with our study as soon as it arrived.
Background
Recent Advances
Drug Development Progress- English Name：
Folate receptor alpha
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
27 Details
- Highest Development Stage：
Approved
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