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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-40 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human FOLR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human FOLR1, His Tag (Cat. No. FO1-H52H1) is more than 95% and the molecular weight of this protein is around 34-55 kDa verified by SEC-MALS.
Immobilized Folic Acid-BSA Conjugate at 5 μg/mL (100 μL/well) can bind Human FOLR1, His Tag (Cat. No. FO1-H52H1) with a linear range of 5-78 ng/mL (QC tested).
Immobilized Human FOLR1, His Tag (Cat. No. FO1-H52H1) at 1 μg/mL (100 μL/well) can bind Anti-FOLR1 Antibody,Mouse IgG1 with a linear range of 0.2-1 ng/mL (QC tested).
Immobilized Anti-FOLR1 Antibody,Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human FOLR1, His Tag (Cat. No. FO1-H52H1) with a linear range of 1-5 ng/mL (Routinely tested).
2e5 of Anti-FOLR1 CAR-293 cells were stained with 100 μL of 0.3 μg/mL of Human FOLR1, His Tag (Cat. No. FO1-H52H1) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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