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Product Details
Synonyms
CD70, CD27LG, TNFSF7, TNFSF7G, CD27L
Source
Human CD27 Ligand Protein, His,Flag Tag (CDL-H52Da) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1) trimer Design.
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 49.3 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD27 Ligand Protein, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD27 Ligand Protein, His,Flag Tag (Cat. No. CDL-H52Da) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD27 Ligand Protein, His,Flag Tag (Cat. No. CDL-H52Da) at 2 μg/mL (100 μL/well) can bind Human CD27, Fc Tag (Cat. No. CD7-H5254) with a linear range of 2-125 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27 Ligand Protein, His,Flag Tag (Cat. No. CDL-H52Da) : Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.42856 μg/mL (Routinely tested).
Protocol
Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 5 μg/mL (100 μL/well) can bind Human CD27 Ligand Protein, His,Flag Tag (Cat. No. CDL-H52Da) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
CD70 antigen
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
37 Details
- Highest Development Stage：
Phase 2 Clinical
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