Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag. The Avi tag (Avitag™) is inserted in-between the polyhistidine tag and flag tag
The protein has a calculated MW of 51.0 kDa. The protein migrates as 47-57 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag (Cat. No. CDL-H82D7) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.
Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag (Cat. No. CDL-H82D7) with a linear range of 0.1-4 ng/mL (QC tested).
Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27, Fc Tag (Cat. No. CD7-H5254) : Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag (Cat. No. CDL-H82D7) binding reactions. The half maximal inhibitory concentration (IC50) is 3.05858 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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