Human NKG2A&CD94 Protein, Mouse IgG2a Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
NC4-H5253-100ug
$395.00
NC4-H5253-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    NKG2A & CD94

  • Source

    Human NKG2A&CD94, Mouse IgG2a Fc Tag (NC4-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 (NKG2A) & Asp 57 - Ile 179 (CD94) (Accession # P26715-1 (NKG2A) & Q13241-1 (CD94)).

  • Molecular Characterization

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 55.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    NKG2A & CD94 SDS-PAGE

    Human NKG2A&CD94, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    NKG2A & CD94 SEC-MALS

    The purity of Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) is more than 90% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     NKG2A & CD94 ELISA

    Immobilized Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-NKG2A (Z270), Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol
  •  NKG2A & CD94 ELISA

    Immobilized Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) at 5 μg/mL (100 μL/well) can bind various dilution ratio of HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE (Routinely tested).

    Protocol

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Background

CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2A/CD159a is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors that inhibits innate immune system activation. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA)-E on target cells.

Recent Advances

 
Drug Development Progress
  • English Name:

    NK cell lectin-like receptor subfamily C member 1

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    6 Details

  • Highest Development Stage:

    Phase 3 Clinical

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