Product Details
Synonyms
NKG2A & CD94
Source
Human NKG2A&CD94, Mouse IgG2a Fc Tag (NC4-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 (NKG2A) & Asp 57 - Ile 179 (CD94) (Accession # P26715-1 (NKG2A) & Q13241-1 (CD94)).
Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 55.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human NKG2A&CD94, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) is more than 90% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-NKG2A (Z270), Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Immobilized Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) at 5 μg/mL (100 μL/well) can bind various dilution ratio of HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
NK cell lectin-like receptor subfamily C member 1
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
6 Details
- Highest Development Stage:
Phase 3 Clinical
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