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Your Position: Home > Protein > Nectin-4 > NE4-H5255

Human Nectin-4 Protein, Fc Tag (MALS verified)

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  • Synonym
  • Source
    Human Nectin-4, Fc Tag(NE4-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).
    Predicted N-terminus: Gly 32
  • Molecular Characterization
    Nectin-4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.5 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Nectin-4 SDS-PAGE

Human Nectin-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Nectin-4 MALS images

The purity of Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) is more than 90% and the molecular weight of this protein is around 117-143 kDa verified by SEC-MALS.

 Nectin-4 ELISA

Immobilized Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with a linear range of 0.313-5 μg/mL (QC tested).

 Nectin-4 ELISA

Immobilized Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Nectin-4 Antibody with a linear range of 0.2-3 ng/mL (QC tested).

 Nectin-4 BLI

Loaded Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 0.263 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Nectin-4 BLI

Loaded Human Nectin-1, His Tag (Cat. No. PV1-H5223) on HIS1K Biosensor, can bind Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) with an affinity constant of 2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Nectin-4(Nectin cell adhesion molecule 4) is a Nectin and nectin-like family of cell adhesion molecules. It is found in high levels in normal embryonic and fetal tissues, but decreases after adulthood and is limited in distribution in healthy tissues. Nectin-4 is overexpressed in a variety of tumor cells and promotes tumor cell growth and proliferation. Solid tumors with high expression of Nectin-4 include bladder cancer, pancreatic cancer, triple negative breast cancer, non-small cell lung cancer, stomach cancer, esophageal cancer, ovarian cancer and many other cancers. Studies have found that Nectin-4 promotes tumor cell proliferation, differentiation, migration and invasion by activating the PI3K/Akt pathway, and is believed to play an important role in the occurrence and metastasis of cancer. It is based on these research backgrounds that drug research targeting this target has emerged.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $350.00

Price(USD) : $2540.00

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