This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 78.6 kDa. The protein migrates as 100-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated SARS-CoV-2 S1 protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) is more than 85% and the molecular weight of this protein is around 125-135 kDa verified by SEC-MALS.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) with a linear range of 0.01-0.625 μg/mL (QC tested).
Immobilized Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.2-3 ng/mL (Routinely tested).
Loaded Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) on SA Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 1.61 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Zupancic JM, Desai AA, Schardt JS, et al.
Journal: Cell chemical biology 2021
Application: Binding Assay
Authors: Ng KW, Attig J, Bolland W, et al.
Journal: Nature genetics 2020
Application: Flow cytometry
Authors: Kaneko N, Satta S, Komuro Y, et al.
Journal: Stroke 2020
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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