This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.5 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein RBD, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) is more than 90% and the molecular weight of this protein is around 110-125 kDa verified by SEC-MALS.
Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) with an affinity constant of 4.48 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Wang C, Wu Z, Liu B, et al.
Journal: Biosens Bioelectron 2021
Application: Binding Assay
Authors: Zupancic JM, Desai AA, Schardt JS, et al.
Journal: Cell chemical biology 2021
Application: Binding Assay
Authors: Anderson, George P et al.
Journal: Analytical chemistry vol 2021
Authors: Miller CJ, McGinnis JE, Martinez MJ et al.
Journal: New biotechnology 2021
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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