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Your Position: Home > Host receptor > ACE2 > AC2-H82E6

Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    ACE-2,ACEH,ACE2
  • Source
    Biotinylated Human ACE2, His,Avitag (AC2-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
    Predicted N-terminus: Gln 18
  • Molecular Characterization
    Online(Gln 18 - Ser 740) Q9BYF1-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 87.2 kDa. The protein migrates as 95-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
SDS-PAGE
Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) SDS-PAGE gel

Biotinylated Human ACE2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human ACE2, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) is more than 90% and the molecular weight of this protein is around 187-229 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human ACE2, His,AvitagBiotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) ELISA bioactivity

Immobilized Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.1-6 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) captured on Biotin CAP - Series S sensor Chip can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 54.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) on SA Biosensor, can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 42.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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ETD of in-stock products: 2 business days

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