This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 87.2 kDa. The protein migrates as 95-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human ACE2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) was more than 90% and around 190-230 kDa verified by SEC-MALS.
Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) captured on Biotin CAP - Series S sensor Chip can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 54.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) captured on Biotin CAP - Series S sensor Chip can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) with an affinity constant of 16.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) on SA Biosensor, can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 42.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) on SA Biosensor, can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) with an affinity constant of 33.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $380.00
Price(USD) : $1570.00
High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed SARS-CoV-2 antigens specifically designed and optimized for serological test kits which are customized formulation available, capable of supplying in bulk order and functionality confirmed.
The affinity constant of SARS-CoV-2 S protein (active trimer) binding to human ACE2 protein was 35.3 nM as verified by SPR.
Binding signal of biotinylated human ACE2 and SARS-CoV-2 S protein RBD was inhibited by SARS-CoV-2 inhibitor with the IC50 10.69 nM.
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