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Your Position: Home > Viral Proteins > S protein RBD > SPD-C82E9

Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)

  • Synonym
    S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,COVID-19
  • Source
    Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (SPD-C82E9) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1).
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Online(Arg 319 - Phe 541) QHD43416.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 28.7 kDa. The protein migrates as 34-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) SDS-PAGE gel

Biotinylated SARS-CoV-2 S protein RBD, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) was more than 90% and around 30-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated SARS-CoV-2 S protein RBD, His,AvitagBiotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) with a linear range of 4-125 ng/mL (QC tested).

Biotinylated SARS-CoV-2 S protein RBD, His,AvitagBiotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) ELISA bioactivity

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) on SA Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 5.86 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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