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Your Position: Home > All Other Proteins > ACE2 > AC2-H52H8

Human ACE2 / ACEH Protein, His Tag (MALS verified)

  • Synonym
    ACE-2,ACEH,ACE2
  • Source
    Human ACE2, His Tag (AC2-H52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Gln 18 - Ser 740) Q9BYF1-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 85.5 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE
Human ACE2, His Tag (Cat. No. AC2-H52H8) SDS-PAGE gel

Human ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human ACE2, His Tag (Cat. No. ) MALS images

The purity of Human ACE2, His Tag (Cat. No. AC2-H52H8) was more than 85% and the molecular weight of this protein is around 195-220 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human ACE2, His TagHuman ACE2, His Tag (Cat. No. AC2-H52H8) ELISA bioactivity

Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) with a linear range of 0.02-0.156 μg/mL (QC tested).

Human ACE2, His TagHuman ACE2, His Tag (Cat. No. AC2-H52H8) ELISA bioactivity

Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.4-13 ng/mL (Routinely tested).

Human ACE2, His TagHuman ACE2, His Tag (Cat. No. AC2-H52H8) ELISA bioactivity

Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) with a linear range of 0.2-5 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) captured on CM5 Chip via anti-human IgG Fc antibodies can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 3.79 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) on AMC Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.53 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) on AR2G Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 11.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $420.00

Price(USD) : $2800.00

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DMF (Drug Master File)

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