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Your Position: Home > All Other Proteins > ACE2 > AC2-H52H8

Human ACE2 / ACEH Protein, His Tag (MALS verified)

  • Synonym
    ACE-2,ACEH,ACE2
  • Source
    Human ACE2, His Tag (AC2-H52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Gln 18 - Ser 740) Q9BYF1-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 85.5 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;

    2. -70°C for 3 months under sterile conditions.

  • Shipping
    This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE
Human ACE2, His Tag (Cat. No. AC2-H52H8) SDS-PAGE gel

Human ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human ACE2, His Tag (Cat. No. ) MALS images

The purity of Human ACE2, His Tag (Cat. No. AC2-H52H8) was more than 85% and the molecular weight of this protein is around 195-220 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human ACE2, His TagHuman ACE2, His Tag (Cat. No. AC2-H52H8) ELISA bioactivity

Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) with a linear range of 0.02-0.156 μg/mL (QC tested).

Human ACE2, His TagHuman ACE2, His Tag (Cat. No. AC2-H52H8) ELISA bioactivity

Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.4-13 ng/mL (Routinely tested).

Human ACE2, His TagHuman ACE2, His Tag (Cat. No. AC2-H52H8) ELISA bioactivity

Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) with a linear range of 0.2-5 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) captured on CM5 Chip via anti-human IgG Fc antibodies can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 3.79 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) on AMC Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.53 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) on AR2G Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 11.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • Background
    Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $420.00

Price(USD) : $2800.00

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