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Your Position: Home > All Other Proteins > ACE2 > AC2-H5257

Human ACE2 / ACEH Protein, Fc Tag (MALS verified)

  • Synonym
    ACE-2,ACEH,ACE2
  • Source
    Human ACE2, Fc Tag (AC2-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
    Predicted N-terminus: Gln 18
  • Molecular Characterization
    Online(Gln 18 - Ser 740) Q9BYF1-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 110.0 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5. Normally glycerol is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ACE2, Fc Tag (Cat. No. AC2-H5257) SDS-PAGE gel

Human ACE2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human ACE2, Fc Tag (Cat. No. ) MALS images

The purity of Human ACE2, Fc Tag (Cat. No. AC2-H5257) was more than 85% and the molecular weight of this protein is around 250-300 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human ACE2, Fc TagHuman ACE2, Fc Tag (Cat. No. AC2-H5257) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) with a linear range of 0.01-0.313 μg/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) with an affinity constant of 67.6 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 16 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) with an affinity constant of 33.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) with an affinity constant of 17.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 26.0 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $400.00

Price(USD) : $2650.00

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