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Your Position: Home > All Other Proteins > uPAR > UPR-H82E7

Biotinylated Human uPAR / PLAUR Protein, His,Avitag™ (MALS verified)

  • Synonym
    uPAR,PLAUR,CD87,MO3
  • Source
    Biotinylated Human uPAR, His,Avitag (UPR-H82E7) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Arg 303 (Accession # Q03405-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    Online(Leu 23 - Arg 303) Q03405-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 35.0 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) SDS-PAGE gel

Biotinylated Human uPAR, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human uPAR, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) was more than 90% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human uPAR, His,AvitagBiotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) ELISA bioactivity

Immobilized Human PLAU, His Tag (Cat. No. PLU-H5229) at 5 μg/mL (100 μL/well) can bind Biotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) with a linear range of 0.3-2 ng/mL (QC tested).

  • Background
    Urokinase plasminogen activator surface receptor (U-PAR) is also known as PLAUR, Monocyte activation antigen Mo3, CD antigen CD87. PLAUR contains three UPAR/Ly6 domains. U-PAR is expressed in neurons of the rolandic area of the brain (at protein level) and is also expressed in the brain. PLAUR / CD87 interacts with MRC2, SRPX2 and SORL1. PLAUR / UPAR acts as a receptor for urokinase plasminogen activator and plays a role in localizing and promoting plasmin formation. U-PAR mediates the proteolysis-independent signal transduction activation effects of U-PA.
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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