SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag

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NUN-C51H9-100ug
$410.00
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NUN-C51H9-1mg (500ug X 2)
$2,945.00
NUN-C51H9
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Synonyms

Nucleocapsid protein, NP, Protein N

Source

SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C51H9) is expressed from E. coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 49.4 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE).

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.