This protein carries a human IgG1 Fc tag at the N-terminus
The protein has a calculated MW of 74.7 kDa. The protein migrates as 120-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD40 Ligand Protein, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD40 Ligand Protein, Fc Tag (Cat. No. CDL-H5269) is more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.
Immobilized Human CD40 Protein, His Tag (Cat. No. CD0-H5228) at 1 μg/mL (100 μL/well) can bind Human CD40 Ligand Protein, Fc Tag (Cat. No. CDL-H5269) with a linear range of 0.2-3 ng/mL (QC tested).
FACS analysis shows that Human CD40 Ligand Protein, Fc Tag (Cat. No. CDL-H5269) can bind to 293T-CD40 cells surface CD40. The concentration of Human CD40 Ligand is 0.3 μg/mL (Routinely tested).
Human CD40 Ligand Protein, Fc Tag (MALS verified) (Cat. No. CDL-H5269) stimulates secretion of IL-6 by human B cells. The ED50 for this effect is 9.68-11.48 ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4 (Routinely tested).
Neutralization assay shows that the cytokine secretion effect of Human CD40 Ligand Protein, Fc Tag (Cat. No. CDL-H5269) was inhibited by increasing concentration of anti-CD40L mAb. The concentration of CD40L used is 50 ng/mL. The EC50 is 0.051-0.058 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $368.00
Price(USD) : $2667.00
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