This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.9 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
SARS S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) was more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) with an affinity constant of 17.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Collins DP, Steer CJ.
Journal: Hepatic medicine : evidence and research 2021
Application: Cell-based assay
Authors: Zupancic JM, Desai AA, Schardt JS, et al.
Journal: Cell chemical biology 2021
Application: Binding Assay
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