This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 85.7 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) with a linear range of 2-78 ng/mL (QC tested).
Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) with an affinity constant of 4.48 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) on Protein A Biosensor, can bind Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) with an affinity constant of 3.06 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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