This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 101.5 kDa. The protein migrates as 130-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S1 protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate,can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.4-6 ng/mL (QC tested).
Loaded SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 3.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Esparza, Thomas J et al.
Journal: Scientific reports 2020
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