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Your Position: Home > Viral Proteins > Nucleocapsid protein > NUN-C81Q6

Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™

  • Synonym
    Nucleocapsid protein,NP,Protein N,COVID-19
  • Source
    Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (NUN-C81Q6) is expressed from E.coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Met 1 - Ala 419) QHO62115.1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 51.1 kDa. The protein migrates as 52 kDa under reducing (R) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (Cat. No. NUN-C81Q6) SDS-PAGE gel

Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated SARS-CoV-2 Nucleocapsid protein, His,AvitagBiotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (Cat. No. NUN-C81Q6) ELISA bioactivity

Immobilized SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (Cat. No. NUN-C81Q6) with a linear range of 0.4-3 ng/mL (QC tested).

  • Background
    Nucleocapsid protein is a most abundant protein of coronavirus. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we identified as the viral nucleocapsid (N) protein. Novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein. The array of diverse functional activities accommodated in the hantaviral N protein goes far beyond to be a static structural protein and makes it an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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