This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein RBD (V367F), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with a linear range of 1-16 ng/mL (QC tested).
Immobilized SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (Routinely tested).
Immobilized SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.195-6.25 ng/mL (Routinely tested).
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with an affinity constant of 4.33 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with an affinity constant of 5.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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