This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein RBD (V367F), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with an affinity constant of 4.33 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with an affinity constant of 5.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Guo Y, Huang L, Zhang G, et al.
Journal: Nature communications 2021
Authors: Esparza, Thomas J et al.
Journal: Scientific reports 2020
Application: Binding Assay
Cross-neutralization antibodies against SARS-CoV-2 and RBD mutations from convalescent patient antibody libraries. BioRxiv preprint. doi: doi: https://doi.org/10.1101/2020.06.06.137513
Authors: Yan Lou, Wenxiang Zhao, Haitao Wei, et al
Journal: BioRxiv 2020
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