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Your Position: Home > Viral Proteins > S protein RBD > SPD-S52H4

SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,COVID-19
  • Source
    SARS-CoV-2 S protein RBD (V367F), His Tag (SPD-S52H4) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1(V367F)).
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Online(Arg 319 - Phe 541) QHD43416.1 (V367F)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) SDS-PAGE gel

SARS-CoV-2 S protein RBD (V367F), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
SARS-CoV-2 S protein RBD (V367F), His TagSARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with a linear range of 1-16 ng/mL (QC tested).

SARS-CoV-2 S protein RBD (V367F), His TagSARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) ELISA bioactivity

Immobilized SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (Routinely tested).

SARS-CoV-2 S protein RBD (V367F), His TagSARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) ELISA bioactivity

Immobilized SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) ) with a linear range of 0.195-6.25 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with an affinity constant of 4.33 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with an affinity constant of 5.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $400.00

Price(USD) : $2600.00

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Datasheet & Documentation

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DMF (Drug Master File)

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