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Your Position: Home > Viral Proteins > NSP1 > NS1-C51H7

SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

  • Synonym
    NSP1,nsp1,Leader protein,Host translation inhibitor nsp1,COVID-19
  • Source
    SARS-CoV-2 NSP1, His Tag (NS1-C51H7) is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Met 1 - Gly 180) YP_009725297.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 NSP1, His Tag (Cat. No. NS1-C51H7) SDS-PAGE gel

SARS-CoV-2 NSP1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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