This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17F, His Tag (Cat. No. ILF-H5244) with an affinity constant of 219 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) on Protein A Biosensor, can bind Human IL-17F, His Tag (Cat. No. ILF-H5244) with an affinity constant of 1.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL-17F, His Tag (Cat. No. ILF-H5244) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 100 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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