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Your Position: Home > All Other Proteins > CD8 alpha & beta > CDA-HF2W4

FITC-Labeled Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    CD8 alpha & beta,CD8A & CD8B
  • Source
    FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-HF2W4) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B)  (Accession # P01732-1 (CD8A) & P10966-1 (CD8B)). It is the FITC labeled form of Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-H52W5).
    Predicted N-terminus: Ser 22 (CD8A) & Leu 22 (CD8B)
  • Molecular Characterization
    Online(Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B) ) P01732-1 (CD8A) & P10966-1 (CD8B)

    FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 19.5 kDa (CD8 alpha) and 22.1 kDa (CD8 beta). Subunit CD8 alpha is fused with a polyhistidine tag at the C-terminus and subunit CD8 beta contains no tag. As a result of glycosylation, the heterodimer protein migrates as 30-32 kDa and 32-34 kDa under reducing (R) condition, and 30-33 kDa and 60-65 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 2-3.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-HF2W4) SDS-PAGE gel

FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag FreeFITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-HF2W4) ELISA bioactivity

Immobilized Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-HF2W4) with a linear range of 0.625-2.5 μg/mL (QC tested).

  • Background
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. CD8A forms disulfide-linked heterodimers with CD8B at the cell surface. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Additionally, CD8B plays a critical role in thymic selection of CD8+ T-cells. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $319.00

Price(USD) : $1980.00

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