0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > CD8 alpha & beta > CDA-H52W5

Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His Tag&Tag Free (MALS verified)

  • Synonym
    CD8 alpha & beta,CD8A & CD8B
  • Source
    Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-H52W5) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B) (Accession # P01732-1(CD8A) & P10966-1(CD8B)).
    Predicted N-terminus: Ser 22 (CD8A) & Leu 22 (CD8B)
  • Molecular Characterization
    Online(Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B) ) P01732-1 (CD8A) & P10966-1 (CD8B)

    Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 19.5 kDa (CD8 alpha) and 22.1 kDa (CD8 beta). Subunit CD8 alpha is fused with a polyhistidine tag at the C-terminus and subunit CD8 beta contains no tag. As a result of glycosylation, the heterodimer protein migrates as 30-32 kDa and 32-34 kDa under reducing (R) condition, and 30-33 kDa and 60-65 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) SDS-PAGE gel

Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. ) MALS images

The purity of Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) was more than 85% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD8A&CD8B Heterodimer Protein, His Tag&Tag FreeHuman CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) ELISA bioactivity

Immobilized Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) at 1 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) with a linear range of 0.2-5 ng/mL (QC tested).

Human CD8A&CD8B Heterodimer Protein, His Tag&Tag FreeHuman CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) ELISA bioactivity

Immobilized Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) at 1 μg/mL (100 μL/well) can bind Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) with a linear range of 2-39 ng/mL (Routinely tested).

  • Background
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. CD8A forms disulfide-linked heterodimers with CD8B at the cell surface. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Additionally, CD8B plays a critical role in thymic selection of CD8+ T-cells. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $380.00

Price(USD) : $2800.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop