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Your Position: Home > Beads > Spike S1 > MBS-K002

SARS-CoV-2 Spike RBD-coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Coupled amount of protein>318 pmol (40 μg) Spike RBD / mg Beads>318 pmol (40 μg) Spike RBD / mg Beads
Capacity>40 μg anti-SARS2-CoV-2 Spike S1 antibody / mg beads; >40 μg ACE2 protein / mg beads >40 μg anti-SARS2-CoV-2 S1 antibody / mg beads; >40 μg ACE2 protein / mg beads
FormulationPBS, 0.05% Tween-20, pH7.4, with 10% TrehalosePBS, 0.05% Tween-20, pH7.4, with 10% Trehalose
Product FormatPowderPowder
Reconstitution2 mL ultrapure water (1 mg beads / mL)Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)
Concentration1 mg beads / mL1 mg beads / mL
  • Background
    The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike RBD protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This very first SARS-CoV-2 Spike protein RBD-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized beads at -20℃ for long term storage. The shelf life is 1 year at -20℃ in lyophilized form.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The Antigen pre-Coupled magnetic beads are Coupled with Biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 Spike protein RBD coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as immunocapture, biopanning and flow cytometry.

    Application Method:

    a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.

    b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.

Typical Data
SARS-CoV-2 Spike RBD-coupled Magnetic BeadsSARS-CoV-2 Spike RBD-coupled Magnetic Beads (Cat. No. MBS-K002) ELISA bioactivity

Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 0.8887 μg/mL (QC tested).

SARS-CoV-2 Spike RBD-coupled Magnetic BeadsSARS-CoV-2 Spike RBD-coupled Magnetic Beads (Cat. No. MBS-K002) ELISA bioactivity

Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.008 μg/mL (QC tested).

SARS-CoV-2 Spike RBD-coupled Magnetic BeadsSARS-CoV-2 Spike RBD-coupled Magnetic Beads (Cat. No. MBS-K002) ELISA bioactivity

The binding curves between SARS-CoV-2 S RBD pre-coupling magnetic beads (Cat. No. MBS-K002) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).

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