| Items | Size （2mg） | Size（5mg X 2） |
|---|---|---|
| Particle size | 2 μm | 2 μm |
| Coupled amount of protein | >318 pmol (40 μg) Spike RBD / mg Beads | >318 pmol (40 μg) Spike RBD / mg Beads |
| Capacity | >40 μg anti-SARS2-CoV-2 Spike S1 antibody / mg beads; >40 μg ACE2 protein / mg beads | >40 μg anti-SARS2-CoV-2 S1 antibody / mg beads; >40 μg ACE2 protein / mg beads |
| Formulation | PBS, 0.05% Tween-20, pH7.4, with 10% Trehalose | PBS, 0.05% Tween-20, pH7.4, with 10% Trehalose |
| Product Format | Powder | Powder |
| Reconstitution | 2 mL ultrapure water (1 mg beads / mL) | Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL) |
| Concentration | 1 mg beads / mL | 1 mg beads / mL |
Upon receipt, please store the lyophilized beads at -20℃ for long term storage. The shelf life is 1 year at -20℃ in lyophilized form.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Application Method:
a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.
Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 0.8887 μg/mL (QC tested).
Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.008 μg/mL (QC tested).
The binding curves between SARS-CoV-2 S RBD pre-coupling magnetic beads (Cat. No. MBS-K002) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
ACROBiosystems has developed an exclusive immune checkpoint protein collection， The consistency and excellent performance of our proteins are confirmed by a variety of assays.
ACROBiosystems has developed a series of homogeneous CD3 proteins with good bioactivity. They are verified as 1:1 heterodimers by nonreductive electrophoresis and MALS as well.
ACROBiosystems has developed a series of products with different species and tags for a variety of hot targets. It is suitable for immune, antibody screening, SPR, cell activity detection and other experiments, and all SPR&BLI protocols are available for free.
We also supply difficult-to-express proteins such as BCMA, CD19, ROR1, and EGFRVIII.
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