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Your Position: Home > All Other Proteins > Mesothelin > MSN-C52H6

Cynomolgus Mesothelin / MSLN (296-580) Protein, His Tag (MALS verified)

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Cynomolgus Mesothelin (296-580), His Tag (MSN-C52H6) is expressed from human 293 cells (HEK293). It contains AA Asp 296 - Gly 580 (Accession # A0A2K5TW94-1).
    Predicted N-terminus: Asp 296
  • Molecular Characterization
    Online(Asp 296 - Gly 580) A0A2K5TW94-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 33.7 kDa. The protein migrates as 35-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) SDS-PAGE gel

Cynomolgus Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus Mesothelin (296-580), His Tag (Cat. No. ) MALS images

The purity of Cynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) was more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus Mesothelin (296-580), His TagCynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) ELISA bioactivity

Immobilized Cynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).

Cynomolgus Mesothelin (296-580), His TagCynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) ELISA bioactivity

Immobilized Cynomolgus Mesothelin (296-580), His Tag (Cat. No. MSN-C52H6) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with a linear range of 2-39 ng/mL (Routinely tested).

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