user
cart
menu
orderQuote Inquiry / OrdercartCart
letter
Sign InRegister
langEnglisharrow
1/12
+9 View all
COADS MSDS

Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free, low endotoxin (Mass Spec & MALS verified)

AIDD‑Ready
Related Products: Cell line SCJUR-STF046, Bridging ELISA Kit
1/12
+9 View all
COADS MSDS
Cat. No. / Size
Price
Availability
Qty
CDD-H52W1-50ug
$435.00
In Stock - Delivery in 1-2 business days
CDD-H52W1-1mg
$4,565.00
In Stock - Delivery in 1-2 business days
CDD-H52W1
Sub-Total :
$ 0
0
Add to Cart
Customize Product Request Quote
Contact Us
Contact Us
Sub-Total:
$ 0
0
Add to Cart
Request Quote
Product Details
Performance Data
Reviews & Citations
Resources
Add to Cart
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

CD3E & CD3D, CD3 delta & CD3 epsilon

Source

Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-H52W1) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).

Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The reducing (R) heterodimer protein migrates as 25-30 kDa and 20 kDa and 22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.

The protein is designed as a heterodimer.

Mass Spectrometry

  • Measured Molecular Mass

    The molecular weight by mass spectrometry is 16760 (CD3E) & 13448 (CD3D) Da (Routinely tested).

  • Sequence Coverage

    Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free(CDD-H52W1) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving CD3E: 100% and CD3D: 100% sequence coverage (Routinely tested).

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.