This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags.
The protein has a calculated MW of 45.5 kDa. As a result of glycosylation, the protein migrates as 55-65 kDa under reducing (R) condition, and 115-130 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human PD-1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human PD-1, Fc,Avitag,His Tag(Cat. No. PD1-H82F4) is more than 90% and the molecular weight of this protein is around 125-140 kDa verified by SEC-MALS.
Immobilized Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) with a linear range of 10-156 ng/mL (QC tested).
Immobilized Nivolumab at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) with a linear range of 0.1-3 ng/mL (Routinely tested).
Serial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258): Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2394 μg/mL (Routinely tested).
Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) (Cat. No. PD1-H5229) with an affinity constant of 1.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) on SA Biosensor, can bind PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.42 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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