This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 17 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 34.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) on SA Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 1.01 μM as determined in BLI assay (ForteBio Octet Red96e)
Authors: Mei, Jie et al.
Journal: Journal of ethnopharmacology 2021
Authors: Guo Y, Huang L, Zhang G, et al.
Journal: Nature communications 2021
Authors: Pratik Adhikary, Sashi Kandel, et al.
Journal: Advanced Therapeutics. 2021
Authors: Narang, Dominic et al.
Journal: Journal of the American Society for Mass Spectrometry 2021
Authors: Sasisekharan, Varun et al.
Journal: Proc Natl Acad Sci U S A 2021
Authors: Wei C, Wan L, Yan Q, et al.
Journal: Nature metabolism 2020
Application: SPR & FACS & Confocal microscopy
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