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Your Position: Home > All Other Proteins > Integrin alpha M beta 2 > IT2-H52W4

Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    Integrin alpha M beta 2,ITGAM&ITGB2
  • Source
    Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-H52W4) is expressed from human 293 cells (HEK293). It contains AA Phe 17 - Asn 1105 (ITGAM) & Gln 23 - Asn 700 (ITGB2) (Accession # P11215-2 (ITGAM) & P05107-1 (ITGB2)).
    Predicted N-terminus: Phe 17 (ITGAM) & Gln 23 (ITGB2)
  • Molecular Characterization
    Online(Phe 17 - Asn 1105 (ITGAM) & Gln 23 - Asn 700 (ITGB2)) P11215-2 (ITGAM) & P05107-1 (ITGB2)

    Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAM and ITGB2, has a calculated MW of 127.2 kDa (ITGAM) and 80.2 kDa (ITGB2). Subunit ITGAM is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 150-180 kDa (ITGAM) and 85-95 kDa (ITGB2) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H52W4) SDS-PAGE gel

Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $410.00

Price(USD) : $2880.00

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