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Your Position: Home > All Other Proteins > CD19 > CD9-H82E9

Biotinylated Human CD19 (20-291) Protein, His,Avitag™ (SPR verified)

  • Synonym
    CD19,B4,CVID3,MGC12802
  • Source
    Biotinylated Human CD19 (20-291), His,Avitag (CD9-H82E9) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).
    Predicted N-terminus: Pro 20
  • Molecular Characterization
    Online(Pro 20 - Lys 291) P15391-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 33.7 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.3-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) SDS-PAGE gel

Biotinylated Human CD19 (20-291), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human CD19 (20-291), His,AvitagBiotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) ELISA bioactivity

Immobilized Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) with a linear range of 1-31 ng/mL (Routinely tested).

Biotinylated Human CD19 (20-291), His,AvitagBiotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) ELISA bioactivity

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) captured on Biotin CAP - Series S sensor Chip can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

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Order Details

Price(USD) : $499.00

Price(USD) : $2380.00

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DMF (Drug Master File)

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