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Your Position: Home > Protein-coupled magnetic beads > CD3E & CD3D > MBS-K003

CD3 epsilon&CD3 delta Heterodimer protein-coupled magnetic beads

For research use only.
Materials Provided
ItemsSize Size
Pack Size2mg10mg
Particle size2 μm2 μm
Beads Surfacehydrophilichydrophilic
Amount of CD3 protein coupled30 μg CD3E & CD3D /mg Beads30 μg CD3E & CD3D /mg Beads
Binding Capacity>200 pmol antibody/ mg beads>200 pmol antibody/ mg beads
FormulationLyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehaloseLyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose
Reconstitution2 mL ultrapure water (1mg beads/mL)10 mL ultrapure water (1mg beads/mL)
  • Background
    The biotinylated CD3E & CD3D protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD3E & CD3D antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This Human CD3E & CD3D coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized beads at -20℃ for long term storage. The shelf life is 1 year at -20℃ in lyophilized form.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The conjugation was achieved by means of the binding between streptavidin and biotin. Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L. Thus, the binding of streptavidin and biotin is irreversible. Our Human CD3E & CD3D-coupled magnetic beads could capture anything binding to CD3E & CD3D, and make the following testing easy, such as immunocapture, biopanning and flow cytometry.

    Application Method:

    a) Reconstitute the Beads following the COA. Wash and re-suspended the beads to a certain concentration by adding your dilution buffer.

    b) Add the prepared beads to your samples.

    c) Beads can be separated from your samples afterwards using a magnetic plate.

Typical Data
CD3 epsilon&CD3 delta Heterodimer protein-coupled magnetic beadsCD3 epsilon&CD3 delta Heterodimer protein-coupled magnetic beads (Cat. No. MBS-K003) ELISA bioactivity

Add CD3 pre-coupling magnetic beads into each well at a concentration of 1 mg/ml. 0.1 mg beads was added to each well. Then add increasing concentrations of anti-CD3 antibody to different wells. PE-labeled anti-human IgG Fc antibody was used for detection (QC tested).

CD3 epsilon&CD3 delta Heterodimer protein-coupled magnetic beadsCD3 epsilon&CD3 delta Heterodimer protein-coupled magnetic beads (Cat. No. MBS-K003) ELISA bioactivity

The binding curves between CD3E&D pre-coupling magnetic beads (Cat. No. MBS-K003) after different freeze-thaw cycles and anti-CD3 antibody. 0.1 mg of Beads (1 mg/mL, 100 μL) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/mL-0.039 μg/mL) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).

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Price(USD) : $600.00

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