|Particle size||2 μm||2 μm|
|Amount of CD3 protein coupled||30 μg CD3E & CD3D /mg Beads||30 μg CD3E & CD3D /mg Beads|
|Binding Capacity||>200 pmol antibody/ mg beads||>200 pmol antibody/ mg beads|
|Formulation||Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose||Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose|
|Reconstitution||2 mL ultrapure water (1mg beads/mL)||10 mL ultrapure water (1mg beads/mL)|
Upon receipt, please store the lyophilized beads at -20℃ for long term storage. The shelf life is 1 year at -20℃ in lyophilized form.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
a) Reconstitute the Beads following the COA. Wash and re-suspended the beads to a certain concentration by adding your dilution buffer.
b) Add the prepared beads to your samples.
c) Beads can be separated from your samples afterwards using a magnetic plate.
Add CD3 pre-coupling magnetic beads into each well at a concentration of 1 mg/ml. 0.1 mg beads was added to each well. Then add increasing concentrations of anti-CD3 antibody to different wells. PE-labeled anti-human IgG Fc antibody was used for detection (QC tested).
The binding curves between CD3E&D pre-coupling magnetic beads (Cat. No. MBS-K003) after different freeze-thaw cycles and anti-CD3 antibody. 0.1 mg of Beads (1 mg/mL, 100 μL) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/mL-0.039 μg/mL) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
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