Human CD3E & CD3D Heterodimer-coupled Magnetic Beads

The biotinylated CD3E & CD3D protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD3E & CD3D antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This Human CD3E & CD3D coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.

This product is intended for immunocapture, biopanning. This is a non-sterile product.

* The Isotype control (Cat. No. SMB-B01) is sold separately.

See Certificate of Analysis (CoA) for detailed instruction.

Upon receipt, please store the lyophilized beads at -20°C for long term storage. The shelf life is 1 year at -20°C in lyophilized form.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.