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Your Position: Home > All Other Proteins > NOTCH1 > NO1-M52H8

Mouse NOTCH1 Protein, His Tag

  • Synonym
    NOTCH1,Notch 1,hN1,TAN1
  • Source
    Mouse NOTCH1, His Tag (NO1-M52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Gln 526 (Accession # Q01705-1).
    Predicted N-terminus: Ala 19
  • Molecular Characterization
    Online(Ala 19 - Gln 526) Q01705-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.4 kDa. The protein migrates as 58-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse NOTCH1, His Tag (Cat. No. NO1-M52H8) SDS-PAGE gel

Mouse NOTCH1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse NOTCH1, His TagMouse NOTCH1, His Tag (Cat. No. NO1-M52H8) ELISA bioactivity

Immobilized Human DLL4, Fc Tag (Cat. No. DL4-H5259) at 5 μg/mL (100 μL/well) can bind Mouse NOTCH1, His Tag (Cat. No. NO1-M52H8) with a linear range of 0.02-0.313 μg/mL (QC tested).

  • Background
    NOTCH1 Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The NOTCH1 intracellular domain interacts with SNW1; the interaction involves multimerized NOTCH1 NICD and is implicated in a formation of an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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