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Your Position: Home > All Other Proteins > APRIL > APL-H52D1

Human APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)

  • Synonym
    APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2
  • Source
    Human APRIL, His,Flag Tag, active trimer (APL-H52D1) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # O75888-1) trimer Design.
    Predicted N-terminus: His
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 50.2 kDa. The protein migrates as 50-55 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) SDS-PAGE gel

Human APRIL, His,Flag Tag, active trimer on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human APRIL, His,Flag Tag, active trimer (Cat. No. ) MALS images

The purity of Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) was more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human APRIL, His,Flag Tag, active trimerHuman APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) ELISA bioactivity

Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) with a linear range of 10-39 ng/mL (QC tested).

Human APRIL, His,Flag Tag, active trimerHuman APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) ELISA bioactivity

Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Human TACI/TNFRSF13B, Fc Tag (Cat. No. TAI-H5256) with a linear range of 0.8-25 ng/mL (Routinely tested).

Human APRIL, His,Flag Tag, active trimerHuman APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) ELISA bioactivity

Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag (Cat. No. BCA-C82E5) with a linear range of 0.2-1 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) on HIS1K Biosensor, can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with an affinity constant of 1.88 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) on HIS1K Biosensor, can bind Human TACI/TNFRSF13B, Fc Tag (Cat. No. TAI-H5256) with an affinity constant of 75 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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