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Your Position: Home > All Other Proteins > IL-2 R beta & IL-2 R gamma > ILG-H5254

Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

  • Synonym
    IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG
  • Source
    Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (ILG-H5254) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # NP_000869.1(IL-2RB) & P31785-1(IL-2RG)).
    Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)
  • Molecular Characterization
    Online(Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG)) NP_000869.1 (IL-2RB) & P31785-1 (IL-2RG)

    Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 51.1 kDa (IL-2RB) and 53.6 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) protein migrates as 65-80 kDa and 90-100 kDa respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) SDS-PAGE gel

Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ) MALS images

The purity of Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) was more than 90% and the molecular weight of this protein is around 145-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) ELISA bioactivity

Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4113) at 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) with a linear range of 5-78 ng/mL (QC tested).

Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) ELISA bioactivity

Immobilized Human IL-15, Tag Free (Cat. No. IL5-H4117) at 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) with a linear range of 0.039-0.625 μg/mL (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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